Dr Fabrice Gielen Senior Lecturer F.Gielen@exeter.ac.uk Living Systems Institute/ Systems Block Groups: Gielen Group University profile 0000-0003-0604-7224 Publications Publications 2025 Harnessing droplet microfluidics and morphology-based deep learning for the label-free study of polymicrobial-phage interactions Published: 24/03/2025 DOI: 10.1101/2025.03.24.644924 URL: https://doi.org/10.1101/2025.03.24.644924 2023 Cancer-associated fibroblasts influence Wnt/PCP signaling in gastric cancer cells by cytoneme-based dissemination of ROR2 Journal: Proceedings of the National Academy of Sciences Published: 26/09/2023 DOI: 10.1073/pnas.2217612120 URL: https://doi.org/10.1073/pnas.2217612120 Investigating bacteria-phage interaction dynamics using droplet-based technology Published: 14/07/2023 DOI: 10.1101/2023.07.14.549014 URL: https://doi.org/10.1101/2023.07.14.549014 Label-free analysis of bacterial growth and lysis at the single-cell level using droplet microfluidics and object detection-oriented deep learning Published: 27/06/2023 DOI: 10.1101/2023.06.27.546533 URL: https://doi.org/10.1101/2023.06.27.546533 2022 High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform Journal: Analytical Chemistry Published: 06/12/2022 DOI: 10.1021/acs.analchem.2c03164 URL: https://doi.org/10.1021/acs.analchem.2c03164 Phenotyping single-cell motility in microfluidic confinement Journal: eLife Published: 23/11/2022 DOI: 10.7554/eLife.76519 URL: https://doi.org/10.7554/eLife.76519 High throughput steady-state enzyme kinetics measured in a parallel droplet generation and absorbance detection platform Published: 31/07/2022 DOI: 10.1101/2022.07.28.500969 URL: https://doi.org/10.1101/2022.07.28.500969 Multi‐Object Detector YOLOv4‐Tiny Enables High‐Throughput Combinatorial and Spatially‐Resolved Sorting of Cells in Microdroplets Journal: Advanced Materials Technologies Published: 30/04/2022 DOI: 10.1002/admt.202101053 ISSN: 2365-709X ISSN: 2365-709X URL: http://dx.doi.org/10.1002/admt.202101053 2021 Materials and methods for droplet microfluidic device fabrication Journal: Lab on a Chip Published: 30/11/2021 DOI: 10.1039/d1lc00836f ISSN: 1473-0197 ISSN: 1473-0189 URL: http://dx.doi.org/10.1039/d1lc00836f Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats Journal: Proceedings of the National Academy of Sciences Published: 16/11/2021 DOI: 10.1073/pnas.2017708118 ISSN: 0027-8424 ISSN: 1091-6490 URL: http://dx.doi.org/10.1073/pnas.2017708118 Functional Phenotype Flow Cytometry: On Chip Sorting of Individual Cells According to Responses to Stimuli. Journal: Advanced Biology Published: 31/07/2021 DOI: 10.1002/adbi.202100220 ISSN: 2701-0198 ISSN: 2701-0198 URL: http://dx.doi.org/10.1002/adbi.202100220 2019 Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures Journal: Lab on a Chip Published: 30/11/2019 DOI: 10.1039/d0lc00055h ISSN: 1473-0197 ISSN: 1473-0189 URL: http://dx.doi.org/10.1039/d0lc00055h Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics Journal: Analytical Chemistry Published: 10/07/2019 DOI: 10.1021/acs.analchem.9b01839 ISSN: 0003-2700 ISSN: 1520-6882 URL: http://dx.doi.org/10.1021/acs.analchem.9b01839 Long‐Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation Journal: Small Published: 31/01/2019 DOI: 10.1002/smll.201804576 ISSN: 1613-6810 ISSN: 1613-6829 URL: http://dx.doi.org/10.1002/smll.201804576 2017 Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics Journal: Methods in Molecular Biology Published: 30/11/2017 DOI: 10.1007/978-1-4939-7366-8_18 ISBN: 9781493973644 ISBN: 9781493973668 ISSN: 1064-3745 ISSN: 1940-6029 URL: http://dx.doi.org/10.1007/978-1-4939-7366-8_18 2016 Exploring sequence space in search of functional enzymes using microfluidic droplets Journal: Current Opinion in Chemical Biology Published: 30/11/2016 DOI: 10.1016/j.cbpa.2017.02.018 WOSUID: WOS:000401782100020 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000401782100020&KeyUID=WOS:000401782100020 Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets Journal: Analytical Chemistry Published: 30/11/2016 DOI: 10.1021/acs.analchem.6b02528 WOSUID: WOS:000392458100012 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000392458100012&KeyUID=WOS:000392458100012 2015 Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS) Journal: Proceedings of the National Academy of Sciences Published: 30/11/2015 DOI: 10.1073/pnas.1606927113 WOSUID: WOS:000388830700007 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000388830700007&KeyUID=WOS:000388830700007 2014 A Method to Quantify FRET Stoichiometry with Phasor Plot Analysis and Acceptor Lifetime Ingrowth Journal: Biophysical Journal Published: 30/11/2014 DOI: 10.1016/j.bpj.2015.01.012 WOSUID: WOS:000350969000002 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000350969000002&KeyUID=WOS:000350969000002 Interfacing Microwells with Nanoliter Compartments: A Sampler Generating High-Resolution Concentration Gradients for Quantitative Biochemical Analyses in Droplets Journal: Analytical Chemistry Published: 30/11/2014 DOI: 10.1021/ac503336g WOSUID: WOS:000347590400064 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000347590400064&KeyUID=WOS:000347590400064 Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics Journal: Nature Communications Published: 30/11/2014 DOI: 10.1038/ncomms10008 WOSUID: WOS:000367567100003 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000367567100003&KeyUID=WOS:000367567100003 2012 A Fully Unsupervised Compartment-on-Demand Platform for Precise Nanoliter Assays of Time-Dependent Steady-State Enzyme Kinetics and Inhibition Journal: Analytical Chemistry Published: 30/11/2012 DOI: 10.1021/ac400480z WOSUID: WOS:000318756100077 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000318756100077&KeyUID=WOS:000318756100077 2011 Controlled one dimensional oscillation of the Belousov-Zhabotinsky reaction confined within microchannels Journal: Rsc Advances Published: 30/11/2011 DOI: 10.1039/c2ra21019c WOSUID: WOS:000306415100007 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000306415100007&KeyUID=WOS:000306415100007 Dielectric Cell Response in Highly Conductive Buffers Journal: Analytical Chemistry Published: 30/11/2011 DOI: 10.1021/ac2022103 WOSUID: WOS:000300470800009 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000300470800009&KeyUID=WOS:000300470800009 2010 A Microdroplet Dilutor for High-Throughput Screening Journal: Nature Chemistry Published: 30/11/2010 DOI: 10.1038/nchem.1046 WOSUID: WOS:000290846300009 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000290846300009&KeyUID=WOS:000290846300009 2009 High-Resolution Local Imaging of Temperature in Dielectrophoretic Platforms Journal: Analytical Chemistry Published: 30/11/2009 DOI: 10.1021/ac101557g WOSUID: WOS:000281306000064 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000281306000064&KeyUID=WOS:000281306000064 2008 Electro-Coalescence of Digitally Controlled Droplets Journal: Analytical Chemistry Published: 30/11/2008 DOI: 10.1021/ac901188n WOSUID: WOS:000269312000029 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000269312000029&KeyUID=WOS:000269312000029 Increasing the Trapping Efficiency of Particles in Microfluidic Planar Platforms by Means of Negative Dielectrophoresis Journal: Journal of Physical Chemistry B Published: 30/11/2008 DOI: 10.1021/jp808897g WOSUID: WOS:000262902600033 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000262902600033&KeyUID=WOS:000262902600033