Dr Stefano Pagliara Associate Professor S.Pagliara@exeter.ac.uk CLES Groups: Pagliara Group University profile 0000-0001-9796-1956 Publications Publications 2022 An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics Journal: Scientific Reports Published: 30/11/2022 DOI: 10.1038/s41598-022-07973-z ISSN: 2045-2322 URL: http://dx.doi.org/10.1038/s41598-022-07973-z Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells. Journal: Communications Biology Published: 30/11/2022 DOI: 10.1038/s42003-022-03336-6 ISSN: 2399-3642 URL: http://dx.doi.org/10.1038/s42003-022-03336-6 Numerical investigation of flexible Purcell-like integrated microfluidic pumps Journal: Journal of Applied Physics Published: 28/10/2022 DOI: 10.1063/5.0109263 URL: https://doi.org/10.1063/5.0109263 Exaggerated trans-membrane charge of ammonium transporters in nutrient-poor marine environments Journal: Open Biology Published: 30/06/2022 DOI: 10.1098/rsob.220041 URL: https://doi.org/10.1098/rsob.220041 Fast bacterial growth reduces antibiotic accumulation and efficacy. Journal: eLife Published: 07/06/2022 DOI: 10.7554/elife.74062 ISSN: 2050-084X DOI: 10.1101/2021.10.18.464851 URL: http://dx.doi.org/10.7554/elife.74062 2021 Nutrient and salt depletion synergistically boosts glucose metabolism in individual bacteria Published: 31/12/2021 PPR: PPR446913 DOI: 10.1101/2022.01.26.477826 URL: http://europepmc.org/abstract/PPR/PPR446913 Bacteriostatic antibiotics promote CRISPR-Cas adaptive immunity by enabling increased spacer acquisition Journal: Cell Host & Microbe Published: 31/12/2021 DOI: 10.1016/j.chom.2021.11.014 ISSN: 1931-3128 URL: http://dx.doi.org/10.1016/j.chom.2021.11.014 CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage Journal: Nature Communications Published: 30/11/2021 DOI: 10.1038/s41467-021-26610-3 ISSN: 2041-1723 URL: http://dx.doi.org/10.1038/s41467-021-26610-3 Fast bacterial growth reduces antibiotic accumulation and efficacy Published: 19/10/2021 DOI: 10.1101/2021.10.18.464851 URL: https://doi.org/10.1101/2021.10.18.464851 Membrane permeability differentiation at the lipid divide Journal: Biorxiv Published: 13/10/2021 DOI: 10.1101/2021.10.12.464042 URL: https://doi.org/10.1101/2021.10.12.464042 Individual bacteria in structured environments rely on phenotypic resistance to phage Journal: PLOS Biology Published: 12/10/2021 DOI: 10.1371/journal.pbio.3001406 URL: https://doi.org/10.1371/journal.pbio.3001406 Persister Escherichia coli Cells Have a Lower Intracellular pH than Susceptible Cells but Maintain Their pH in Response to Antibiotic Treatment Journal: mBio Published: 31/08/2021 DOI: 10.1128/mbio.00909-21 ISSN: 2150-7511 URL: http://dx.doi.org/10.1128/mbio.00909-21 Editorial: Probing the Chromatin Architecture Journal: Frontiers in Cell and Developmental Biology Published: 29/07/2021 DOI: 10.3389/fcell.2021.727803 ISSN: 2296-634X URL: http://dx.doi.org/10.3389/fcell.2021.727803 Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation Journal: ACS Nano Published: 22/06/2021 DOI: 10.1021/acsnano.1c00218 ISSN: 1936-0851 ISSN: 1936-086X URL: http://dx.doi.org/10.1021/acsnano.1c00218 Heterologous Protein Expression Favors the Formation of Protein Aggregates in Persister and Viable but Nonculturable Bacteria. Journal: ACS Infectious Diseases Published: 11/06/2021 DOI: 10.1021/acsinfecdis.1c00154 URL: https://doi.org/10.1021/acsinfecdis.1c00154 Heterologous Protein Expression Favors the Formation of Protein Aggregates in Persister and Viable but Nonculturable Bacteria Journal: ACS Infectious Diseases Published: 17/05/2021 DOI: 10.1021/acsinfecdis.1c00154 ISSN: 2373-8227 ISSN: 2373-8227 URL: http://dx.doi.org/10.1021/acsinfecdis.1c00154 Single Cell Label-Free Probing of Chromatin Dynamics During B Lymphocyte Maturation. Journal: Frontiers in cell and developmental biology Published: 28/02/2021 PMID: 33842468 PMC: PMC8033168 DOI: 10.3389/fcell.2021.646616 URL: http://europepmc.org/abstract/med/33842468 2020 Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials Journal: Methods in Molecular Biology Published: 30/11/2020 DOI: 10.1007/978-1-0716-0928-6_16 ISBN: 9781071609279 ISBN: 9781071609286 ISSN: 1064-3745 ISSN: 1940-6029 URL: http://dx.doi.org/10.1007/978-1-0716-0928-6_16 Effect of DMSO on the Mechanical and Structural Properties of Model and Biological Membranes Journal: Biophysical Journal Published: 30/06/2020 DOI: 10.1016/j.bpj.2020.05.037 ISSN: 0006-3495 URL: http://dx.doi.org/10.1016/j.bpj.2020.05.037 Waterborne Electrospinning of α-Lactalbumin Generates Tunable and Biocompatible Nanofibers for Drug Delivery Journal: ACS Applied Nano Materials Published: 28/02/2020 DOI: 10.1021/acsanm.9b02557 URL: https://doi.org/10.1021/acsanm.9b02557 Engineering Chirally Blind Protein Pseudocapsids into Antibacterial Persisters Journal: ACS Nano Published: 25/02/2020 DOI: 10.1021/acsnano.9b06814 URL: https://doi.org/10.1021/acsnano.9b06814 2019 Fluorescent macrolide probes – synthesis and use in evaluation of bacterial resistance Journal: RSC Chemical Biology Published: 30/11/2019 DOI: 10.1039/D0CB00118J URL: https://doi.org/10.1039/D0CB00118J Microfluidic devices powered by integrated elasto-magnetic pumps Journal: Lab on a Chip Published: 30/11/2019 DOI: 10.1039/D0LC00935K URL: https://doi.org/10.1039/D0LC00935K Single-cell microfluidics facilitates the rapid quantification of antibiotic accumulation in Gram-negative bacteria Journal: Lab on a Chip Published: 30/11/2019 DOI: 10.1039/D0LC00242A URL: https://doi.org/10.1039/D0LC00242A Single-cell microfluidics facilitates the rapid quantification of antibiotic accumulation in Gram-negative bacteria. Journal: Lab on a Chip Published: 30/11/2019 DOI: 10.1039/D0LC00242A URL: https://doi.org/10.1039/D0LC00242A Bacterial ageing in the absence of external stressors Journal: Philosophical Transactions of the Royal Society B: Biological Sciences Published: 25/11/2019 DOI: 10.1098/rstb.2018.0442 URL: https://doi.org/10.1098/rstb.2018.0442 Single cell ecology Journal: Philosophical Transactions of the Royal Society B: Biological Sciences Published: 25/11/2019 DOI: 10.1098/rstb.2019.0076 URL: https://doi.org/10.1098/rstb.2019.0076 Antibiotic-derived molecular probes for bacterial imaging Journal: Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II Published: 07/03/2019 DOI: 10.1117/12.2507329 URL: http://dx.doi.org/10.1117/12.2507329 2018 Advanced Processing of Micropatterned Elasto-Magnetic Membranes Journal: IEEE Transactions on Magnetics Published: 30/11/2018 DOI: 10.1109/TMAG.2018.2887347 WOSUID: WOS:000472562100001 URL: https://publons.com/publon/26968868/ MMHelper: An automated framework for the analysis of microscopy images acquired with the mother machine Journal: Scientific Reports Published: 30/11/2018 DOI: 10.1038/S41598-019-46567-0 WOSUID: WOS:000475293200025 URL: https://publons.com/publon/20985526/ Single Cell Imaging of Nuclear Architecture Changes Journal: Frontiers in Cell and Developmental Biology Published: 30/11/2018 DOI: 10.3389/FCELL.2019.00141 WOSUID: WOS:000476946200002 URL: https://publons.com/publon/26968869/ 2017 The Culture Environment Influences Both Gene Regulation and Phenotypic Heterogeneity in Escherichia coli Journal: Frontiers in Microbiology Published: 30/11/2017 DOI: 10.3389/FMICB.2018.01739 WOSUID: WOS:000441566100001 URL: https://publons.com/publon/17764384/ Versatile Multiplexed Super-Resolution Imaging of Nanostructures by Quencher-Exchange-Paint Journal: Biophysical Journal Published: 30/11/2017 DOI: 10.1007/S12274-018-1971-6 WOSUID: WOS:000430450000243 URL: https://publons.com/publon/17764383/ Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy Journal: BMC Biology Published: 30/11/2017 DOI: 10.1186/s12915-017-0465-4 URL: https://doi.org/10.1186/s12915-017-0465-4 Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy. Journal: BMC Biology Published: 30/11/2017 DOI: 10.1186/s12915-017-0465-4 URL: https://doi.org/10.1186/s12915-017-0465-4 2016 A microfluidic device for characterizing nuclear deformations Journal: Lab on a Chip Published: 30/11/2016 DOI: 10.1039/C6LC01308B WOSUID: WOS:000395893500005 URL: https://publons.com/publon/11342592/ Particle transport across a channel via an oscillating potential Journal: Physical Review E Published: 30/11/2016 DOI: 10.1103/PHYSREVE.96.052401 WOSUID: WOS:000414135000005 URL: https://publons.com/publon/11342591/ 2015 Direct Optofluidic Measurement of the Lipid Permeability of Fluoroquinolones Journal: Scientific Reports Published: 30/11/2015 DOI: 10.1038/SREP32824 WOSUID: WOS:000382644200001 URL: https://publons.com/publon/3686885/ Single-File Escape of Colloidal Particles from Microfluidic Channels Journal: Physical Review Letters Published: 30/11/2015 DOI: 10.1103/PHYSREVLETT.117.038001 WOSUID: WOS:000379514500017 URL: https://publons.com/publon/11342593/ 2014 Nondecaying Hydrodynamic Interactions along Narrow Channels Journal: Physical Review Letters Published: 30/11/2014 DOI: 10.1103/PhysRevLett.115.038301 WOSUID: WOS:000358024500011 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000358024500011&KeyUID=WOS:000358024500011 Quantification of Fluoroquinolone Uptake through the Outer Membrane Channel OmpF of Escherichia coil Journal: Journal of the American Chemical Society Published: 30/11/2014 DOI: 10.1021/jacs.5b08960 WOSUID: WOS:000364355900025 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000364355900025&KeyUID=WOS:000364355900025 Real-time deformability cytometry: on-the-fly cell mechanical phenotyping Journal: Nature Methods Published: 30/11/2014 DOI: 10.1038/nmeth.3281 WOSUID: WOS:000350670300015 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000350670300015&KeyUID=WOS:000350670300015 Diffusion coefficients and particle transport in synthetic membrane channels Journal: The European Physical Journal Special Topics Published: 30/11/2014 DOI: 10.1140/epjst/e2014-02324-6 ISSN: 1951-6355 ISSN: 1951-6401 URL: http://dx.doi.org/10.1140/epjst/e2014-02324-6 2013 A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes Journal: Lab on a Chip Published: 30/11/2013 DOI: 10.1039/c4lc00217b URL: Anisotropic diffusion of spherical particles in closely confining microchannels Journal: Physical Review E Published: 30/11/2013 DOI: 10.1103/PhysRevE.89.062305 URL: Auxetic nuclei in embryonic stem cells exiting pluripotency Journal: Nature Materials Published: 30/11/2013 DOI: 10.1038/NMAT3943 URL: Channel-Facilitated Diffusion Boosted by Particle Binding at the Channel Entrance Journal: Physical Review Letters Published: 30/11/2013 DOI: 10.1103/PhysRevLett.113.048102 URL: Local characterization of hindered Brownian motion by using digital video microscopy and 3D particle tracking Journal: Review of Scientific Instruments Published: 30/11/2013 DOI: 10.1063/1.4865552 URL: Selective transport control on molecular velcro made from intrinsically disordered proteins Journal: Nature Nanotechnology Published: 30/11/2013 DOI: 10.1038/NNANO.2014.103 URL: 2012 Local Mechanical Properties of Electrospun Fibers Correlate to Their Internal Nanostructure Journal: Nano Letters Published: 30/11/2012 DOI: 10.1021/nl4033439 URL: Optimizing Diffusive Transport Through a Synthetic Membrane Channel Journal: Advanced Materials Published: 30/11/2012 DOI: 10.1002/adma.201203500 URL: 2011 Colloid Flow Control in Microchannels and Detection by Laser Scattering Journal: UK Colloids 2011 Published: 30/11/2011 DOI: 10.1007/978-3-642-28974-3_9 ISBN: 9783642289736 ISBN: 9783642289743 URL: http://dx.doi.org/10.1007/978-3-642-28974-3_9 Electrical properties of in vitro biomineralized recombinant silicatein deposited by microfluidics Journal: Applied Physics Letters Published: 30/11/2011 DOI: 10.1063/1.4766186 URL: Microfluidics Reveals a Flow-Induced Large-Scale Polymorphism of Protein Aggregates Journal: Journal of Physical Chemistry Letters Published: 30/11/2011 DOI: 10.1021/jz301375d URL: Optical properties of in-vitro biomineralised silica Journal: Scientific Reports Published: 30/11/2011 DOI: 10.1038/srep00607 URL: Perpendicular coupling to in-plane photonics using arc waveguides fabricated via two-photon polymerization Journal: Applied Physics Letters Published: 30/11/2011 DOI: 10.1063/1.4704358 URL: Viscoelastic Properties of Differentiating Blood Cells Are Fate- and Function-Dependent Journal: Plos One Published: 30/11/2011 DOI: 10.1371/journal.pone.0045237 URL: 2010 An electrospun fiber phototransistor by the conjugated polymer poly[2-methoxy-5-(2 ‘-ethylhexyloxy)-1,4-phenylene-vinylene] Journal: Applied Physics Letters Published: 30/11/2010 DOI: 10.1063/1.3534803 URL: Biosilica Electrically-Insulating Layers by Soft Lithography-Assisted Biomineralisation with Recombinant Silicatein Journal: Advanced Materials Published: 30/11/2010 DOI: 10.1002/adma.201102691 URL: Flow-induced polymorphism of protein aggregates in micro channels Journal: European Biophysics Journal With Biophysics Letters Published: 30/11/2010 WOSUID: WOS:000293637300180 URL: https://publons.com/publon/6197534/ Optical Anisotropy in Single Light-Emitting Polymer Nanofibers Journal: Journal of Physical Chemistry C Published: 30/11/2010 DOI: 10.1021/jp204582j URL: Parallel sub-micrometre channels with different dimensions for laser scattering detection Journal: Lab on a Chip Published: 30/11/2010 DOI: 10.1039/c1lc20399a URL: Soft Nanolithography by Polymer Fibers Journal: Advanced Functional Materials Published: 30/11/2010 DOI: 10.1002/adfm.201001901 URL: Silicon Derivate Layers/Films Produced by Silicatein-Mediated Templating and Process for Making the Same Published: 11/05/2010 URL: https://patents.google.com/patent/US20110281077A1/en 2009 Collagen-functionalised electrospun polymer fibers for bioengineering applications Journal: Soft Matter Published: 30/11/2009 DOI: 10.1039/b921932c URL: Enhancement of light polarization from electrospun polymer fibers by room temperature nanoimprint lithography Journal: Nanotechnology Published: 30/11/2009 DOI: 10.1088/0957-4484/21/21/215304 URL: Rotational dynamics of optically trapped nanofibers Journal: Optics Express Published: 30/11/2009 DOI: 10.1364/OE.18.000822 URL: Single light-emitting polymer nanofiber field-effect transistors Journal: Nanoscale Published: 30/11/2009 DOI: 10.1039/c0nr00181c URL: Study of optical properties of electrospun light-emitting polymer fibers Journal: Superlattices and Microstructures Published: 30/11/2009 DOI: 10.1016/j.spmi.2009.07.032 URL: 2008 Electrospun light-emitting nanofibers as excitation source in microfluidic devices Journal: Lab on a Chip Published: 30/11/2008 DOI: 10.1039/b906188f URL: Hierarchical assembly of light-emitting polymer nanofibers in helical morphologies Journal: Applied Physics Letters Published: 30/11/2008 DOI: 10.1063/1.3275727 URL: 2007 Patterning of light-emitting conjugated polymer nanofibres Journal: Nature Nanotechnology Published: 30/11/2007 DOI: 10.1038/nnano.2008.232 URL: 2006 Registration accuracy in multilevel soft lithography Journal: Nanotechnology Published: 30/11/2006 DOI: 10.1088/0957-4484/18/17/175302 URL: