Dr Stefano Pagliara

Associate Professor

S.Pagliara@exeter.ac.uk

CLES

Groups: Pagliara Group

University profile

0000-0001-9796-1956

Publications

2022

An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics

Journal: Scientific Reports

Published: 30/11/2022
DOI: 10.1038/s41598-022-07973-z
ISSN: 2045-2322
URL: http://dx.doi.org/10.1038/s41598-022-07973-z

Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells.

Journal: Communications Biology

Published: 30/11/2022
DOI: 10.1038/s42003-022-03336-6
ISSN: 2399-3642
URL: http://dx.doi.org/10.1038/s42003-022-03336-6

Numerical investigation of flexible Purcell-like integrated microfluidic pumps

Journal: Journal of Applied Physics

Published: 28/10/2022
DOI: 10.1063/5.0109263
URL: https://doi.org/10.1063/5.0109263

Exaggerated trans-membrane charge of ammonium transporters in nutrient-poor marine environments

Journal: Open Biology

Published: 30/06/2022
DOI: 10.1098/rsob.220041
URL: https://doi.org/10.1098/rsob.220041

Fast bacterial growth reduces antibiotic accumulation and efficacy.

Journal: eLife

Published: 07/06/2022
DOI: 10.7554/elife.74062
ISSN: 2050-084X
DOI: 10.1101/2021.10.18.464851
URL: http://dx.doi.org/10.7554/elife.74062

2021

Nutrient and salt depletion synergistically boosts glucose metabolism in individual bacteria

Published: 31/12/2021
PPR: PPR446913
DOI: 10.1101/2022.01.26.477826
URL: http://europepmc.org/abstract/PPR/PPR446913

Bacteriostatic antibiotics promote CRISPR-Cas adaptive immunity by enabling increased spacer acquisition

Journal: Cell Host & Microbe

Published: 31/12/2021
DOI: 10.1016/j.chom.2021.11.014
ISSN: 1931-3128
URL: http://dx.doi.org/10.1016/j.chom.2021.11.014

CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage

Journal: Nature Communications

Published: 30/11/2021
DOI: 10.1038/s41467-021-26610-3
ISSN: 2041-1723
URL: http://dx.doi.org/10.1038/s41467-021-26610-3

Fast bacterial growth reduces antibiotic accumulation and efficacy

Published: 19/10/2021
DOI: 10.1101/2021.10.18.464851
URL: https://doi.org/10.1101/2021.10.18.464851

Membrane permeability differentiation at the lipid divide

Journal: Biorxiv

Published: 13/10/2021
DOI: 10.1101/2021.10.12.464042
URL: https://doi.org/10.1101/2021.10.12.464042

Individual bacteria in structured environments rely on phenotypic resistance to phage

Journal: PLOS Biology

Published: 12/10/2021
DOI: 10.1371/journal.pbio.3001406
URL: https://doi.org/10.1371/journal.pbio.3001406

Persister Escherichia coli Cells Have a Lower Intracellular pH than Susceptible Cells but Maintain Their pH in Response to Antibiotic Treatment

Journal: mBio

Published: 31/08/2021
DOI: 10.1128/mbio.00909-21
ISSN: 2150-7511
URL: http://dx.doi.org/10.1128/mbio.00909-21

Editorial: Probing the Chromatin Architecture

Journal: Frontiers in Cell and Developmental Biology

Published: 29/07/2021
DOI: 10.3389/fcell.2021.727803
ISSN: 2296-634X
URL: http://dx.doi.org/10.3389/fcell.2021.727803

Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation

Journal: ACS Nano

Published: 22/06/2021
DOI: 10.1021/acsnano.1c00218
ISSN: 1936-0851
ISSN: 1936-086X
URL: http://dx.doi.org/10.1021/acsnano.1c00218

Heterologous Protein Expression Favors the Formation of Protein Aggregates in Persister and Viable but Nonculturable Bacteria.

Journal: ACS Infectious Diseases

Published: 11/06/2021
DOI: 10.1021/acsinfecdis.1c00154
URL: https://doi.org/10.1021/acsinfecdis.1c00154

Heterologous Protein Expression Favors the Formation of Protein Aggregates in Persister and Viable but Nonculturable Bacteria

Journal: ACS Infectious Diseases

Published: 17/05/2021
DOI: 10.1021/acsinfecdis.1c00154
ISSN: 2373-8227
ISSN: 2373-8227
URL: http://dx.doi.org/10.1021/acsinfecdis.1c00154

Single Cell Label-Free Probing of Chromatin Dynamics During B Lymphocyte Maturation.

Journal: Frontiers in cell and developmental biology

Published: 28/02/2021
PMID: 33842468
PMC: PMC8033168
DOI: 10.3389/fcell.2021.646616
URL: http://europepmc.org/abstract/med/33842468

2020

Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials

Journal: Methods in Molecular Biology

Published: 30/11/2020
DOI: 10.1007/978-1-0716-0928-6_16
ISBN: 9781071609279
ISBN: 9781071609286
ISSN: 1064-3745
ISSN: 1940-6029
URL: http://dx.doi.org/10.1007/978-1-0716-0928-6_16

Effect of DMSO on the Mechanical and Structural Properties of Model and Biological Membranes

Journal: Biophysical Journal

Published: 30/06/2020
DOI: 10.1016/j.bpj.2020.05.037
ISSN: 0006-3495
URL: http://dx.doi.org/10.1016/j.bpj.2020.05.037

Waterborne Electrospinning of α-Lactalbumin Generates Tunable and Biocompatible Nanofibers for Drug Delivery

Journal: ACS Applied Nano Materials

Published: 28/02/2020
DOI: 10.1021/acsanm.9b02557
URL: https://doi.org/10.1021/acsanm.9b02557

Engineering Chirally Blind Protein Pseudocapsids into Antibacterial Persisters

Journal: ACS Nano

Published: 25/02/2020
DOI: 10.1021/acsnano.9b06814
URL: https://doi.org/10.1021/acsnano.9b06814

2019

Fluorescent macrolide probes – synthesis and use in evaluation of bacterial resistance

Journal: RSC Chemical Biology

Published: 30/11/2019
DOI: 10.1039/D0CB00118J
URL: https://doi.org/10.1039/D0CB00118J

Microfluidic devices powered by integrated elasto-magnetic pumps

Journal: Lab on a Chip

Published: 30/11/2019
DOI: 10.1039/D0LC00935K
URL: https://doi.org/10.1039/D0LC00935K

Single-cell microfluidics facilitates the rapid quantification of antibiotic accumulation in Gram-negative bacteria

Journal: Lab on a Chip

Published: 30/11/2019
DOI: 10.1039/D0LC00242A
URL: https://doi.org/10.1039/D0LC00242A

Single-cell microfluidics facilitates the rapid quantification of antibiotic accumulation in Gram-negative bacteria.

Journal: Lab on a Chip

Published: 30/11/2019
DOI: 10.1039/D0LC00242A
URL: https://doi.org/10.1039/D0LC00242A

Bacterial ageing in the absence of external stressors

Journal: Philosophical Transactions of the Royal Society B: Biological Sciences

Published: 25/11/2019
DOI: 10.1098/rstb.2018.0442
URL: https://doi.org/10.1098/rstb.2018.0442

Single cell ecology

Journal: Philosophical Transactions of the Royal Society B: Biological Sciences

Published: 25/11/2019
DOI: 10.1098/rstb.2019.0076
URL: https://doi.org/10.1098/rstb.2019.0076

Antibiotic-derived molecular probes for bacterial imaging

Journal: Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II

Published: 07/03/2019
DOI: 10.1117/12.2507329
URL: http://dx.doi.org/10.1117/12.2507329

2018

Advanced Processing of Micropatterned Elasto-Magnetic Membranes

Journal: IEEE Transactions on Magnetics

Published: 30/11/2018
DOI: 10.1109/TMAG.2018.2887347
WOSUID: WOS:000472562100001
URL: https://publons.com/publon/26968868/

MMHelper: An automated framework for the analysis of microscopy images acquired with the mother machine

Journal: Scientific Reports

Published: 30/11/2018
DOI: 10.1038/S41598-019-46567-0
WOSUID: WOS:000475293200025
URL: https://publons.com/publon/20985526/

Single Cell Imaging of Nuclear Architecture Changes

Journal: Frontiers in Cell and Developmental Biology

Published: 30/11/2018
DOI: 10.3389/FCELL.2019.00141
WOSUID: WOS:000476946200002
URL: https://publons.com/publon/26968869/

2017

The Culture Environment Influences Both Gene Regulation and Phenotypic Heterogeneity in Escherichia coli

Journal: Frontiers in Microbiology

Published: 30/11/2017
DOI: 10.3389/FMICB.2018.01739
WOSUID: WOS:000441566100001
URL: https://publons.com/publon/17764384/

Versatile Multiplexed Super-Resolution Imaging of Nanostructures by Quencher-Exchange-Paint

Journal: Biophysical Journal

Published: 30/11/2017
DOI: 10.1007/S12274-018-1971-6
WOSUID: WOS:000430450000243
URL: https://publons.com/publon/17764383/

Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy

Journal: BMC Biology

Published: 30/11/2017
DOI: 10.1186/s12915-017-0465-4
URL: https://doi.org/10.1186/s12915-017-0465-4

Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy.

Journal: BMC Biology

Published: 30/11/2017
DOI: 10.1186/s12915-017-0465-4
URL: https://doi.org/10.1186/s12915-017-0465-4

2016

A microfluidic device for characterizing nuclear deformations

Journal: Lab on a Chip

Published: 30/11/2016
DOI: 10.1039/C6LC01308B
WOSUID: WOS:000395893500005
URL: https://publons.com/publon/11342592/

Particle transport across a channel via an oscillating potential

Journal: Physical Review E

Published: 30/11/2016
DOI: 10.1103/PHYSREVE.96.052401
WOSUID: WOS:000414135000005
URL: https://publons.com/publon/11342591/

2015

Direct Optofluidic Measurement of the Lipid Permeability of Fluoroquinolones

Journal: Scientific Reports

Published: 30/11/2015
DOI: 10.1038/SREP32824
WOSUID: WOS:000382644200001
URL: https://publons.com/publon/3686885/

Single-File Escape of Colloidal Particles from Microfluidic Channels

Journal: Physical Review Letters

Published: 30/11/2015
DOI: 10.1103/PHYSREVLETT.117.038001
WOSUID: WOS:000379514500017
URL: https://publons.com/publon/11342593/

2014

Nondecaying Hydrodynamic Interactions along Narrow Channels

Journal: Physical Review Letters

Published: 30/11/2014
DOI: 10.1103/PhysRevLett.115.038301
WOSUID: WOS:000358024500011
URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000358024500011&KeyUID=WOS:000358024500011

Quantification of Fluoroquinolone Uptake through the Outer Membrane Channel OmpF of Escherichia coil

Journal: Journal of the American Chemical Society

Published: 30/11/2014
DOI: 10.1021/jacs.5b08960
WOSUID: WOS:000364355900025
URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000364355900025&KeyUID=WOS:000364355900025

Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

Journal: Nature Methods

Published: 30/11/2014
DOI: 10.1038/nmeth.3281
WOSUID: WOS:000350670300015
URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000350670300015&KeyUID=WOS:000350670300015

Diffusion coefficients and particle transport in synthetic membrane channels

Journal: The European Physical Journal Special Topics

Published: 30/11/2014
DOI: 10.1140/epjst/e2014-02324-6
ISSN: 1951-6355
ISSN: 1951-6401
URL: http://dx.doi.org/10.1140/epjst/e2014-02324-6

2013

A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes

Journal: Lab on a Chip

Published: 30/11/2013
DOI: 10.1039/c4lc00217b
URL:

Anisotropic diffusion of spherical particles in closely confining microchannels

Journal: Physical Review E

Published: 30/11/2013
DOI: 10.1103/PhysRevE.89.062305
URL:

Auxetic nuclei in embryonic stem cells exiting pluripotency

Journal: Nature Materials

Published: 30/11/2013
DOI: 10.1038/NMAT3943
URL:

Channel-Facilitated Diffusion Boosted by Particle Binding at the Channel Entrance

Journal: Physical Review Letters

Published: 30/11/2013
DOI: 10.1103/PhysRevLett.113.048102
URL:

Local characterization of hindered Brownian motion by using digital video microscopy and 3D particle tracking

Journal: Review of Scientific Instruments

Published: 30/11/2013
DOI: 10.1063/1.4865552
URL:

Selective transport control on molecular velcro made from intrinsically disordered proteins

Journal: Nature Nanotechnology

Published: 30/11/2013
DOI: 10.1038/NNANO.2014.103
URL:

2012

Local Mechanical Properties of Electrospun Fibers Correlate to Their Internal Nanostructure

Journal: Nano Letters

Published: 30/11/2012
DOI: 10.1021/nl4033439
URL:

Optimizing Diffusive Transport Through a Synthetic Membrane Channel

Journal: Advanced Materials

Published: 30/11/2012
DOI: 10.1002/adma.201203500
URL:

2011

Colloid Flow Control in Microchannels and Detection by Laser Scattering

Journal: UK Colloids 2011

Published: 30/11/2011
DOI: 10.1007/978-3-642-28974-3_9
ISBN: 9783642289736
ISBN: 9783642289743
URL: http://dx.doi.org/10.1007/978-3-642-28974-3_9

Electrical properties of in vitro biomineralized recombinant silicatein deposited by microfluidics

Journal: Applied Physics Letters

Published: 30/11/2011
DOI: 10.1063/1.4766186
URL:

Microfluidics Reveals a Flow-Induced Large-Scale Polymorphism of Protein Aggregates

Journal: Journal of Physical Chemistry Letters

Published: 30/11/2011
DOI: 10.1021/jz301375d
URL:

Optical properties of in-vitro biomineralised silica

Journal: Scientific Reports

Published: 30/11/2011
DOI: 10.1038/srep00607
URL:

Perpendicular coupling to in-plane photonics using arc waveguides fabricated via two-photon polymerization

Journal: Applied Physics Letters

Published: 30/11/2011
DOI: 10.1063/1.4704358
URL:

Viscoelastic Properties of Differentiating Blood Cells Are Fate- and Function-Dependent

Journal: Plos One

Published: 30/11/2011
DOI: 10.1371/journal.pone.0045237
URL:

2010

An electrospun fiber phototransistor by the conjugated polymer poly[2-methoxy-5-(2 ‘-ethylhexyloxy)-1,4-phenylene-vinylene]

Journal: Applied Physics Letters

Published: 30/11/2010
DOI: 10.1063/1.3534803
URL:

Biosilica Electrically-Insulating Layers by Soft Lithography-Assisted Biomineralisation with Recombinant Silicatein

Journal: Advanced Materials

Published: 30/11/2010
DOI: 10.1002/adma.201102691
URL:

Flow-induced polymorphism of protein aggregates in micro channels

Journal: European Biophysics Journal With Biophysics Letters

Published: 30/11/2010
WOSUID: WOS:000293637300180
URL: https://publons.com/publon/6197534/

Optical Anisotropy in Single Light-Emitting Polymer Nanofibers

Journal: Journal of Physical Chemistry C

Published: 30/11/2010
DOI: 10.1021/jp204582j
URL:

Parallel sub-micrometre channels with different dimensions for laser scattering detection

Journal: Lab on a Chip

Published: 30/11/2010
DOI: 10.1039/c1lc20399a
URL:

Soft Nanolithography by Polymer Fibers

Journal: Advanced Functional Materials

Published: 30/11/2010
DOI: 10.1002/adfm.201001901
URL:

Silicon Derivate Layers/Films Produced by Silicatein-Mediated Templating and Process for Making the Same

Published: 11/05/2010
URL: https://patents.google.com/patent/US20110281077A1/en

2009

Collagen-functionalised electrospun polymer fibers for bioengineering applications

Journal: Soft Matter

Published: 30/11/2009
DOI: 10.1039/b921932c
URL:

Enhancement of light polarization from electrospun polymer fibers by room temperature nanoimprint lithography

Journal: Nanotechnology

Published: 30/11/2009
DOI: 10.1088/0957-4484/21/21/215304
URL:

Rotational dynamics of optically trapped nanofibers

Journal: Optics Express

Published: 30/11/2009
DOI: 10.1364/OE.18.000822
URL:

Single light-emitting polymer nanofiber field-effect transistors

Journal: Nanoscale

Published: 30/11/2009
DOI: 10.1039/c0nr00181c
URL:

Study of optical properties of electrospun light-emitting polymer fibers

Journal: Superlattices and Microstructures

Published: 30/11/2009
DOI: 10.1016/j.spmi.2009.07.032
URL:

2008

Electrospun light-emitting nanofibers as excitation source in microfluidic devices

Journal: Lab on a Chip

Published: 30/11/2008
DOI: 10.1039/b906188f
URL:

Hierarchical assembly of light-emitting polymer nanofibers in helical morphologies

Journal: Applied Physics Letters

Published: 30/11/2008
DOI: 10.1063/1.3275727
URL:

2007

Patterning of light-emitting conjugated polymer nanofibres

Journal: Nature Nanotechnology

Published: 30/11/2007
DOI: 10.1038/nnano.2008.232
URL:

2006

Registration accuracy in multilevel soft lithography

Journal: Nanotechnology

Published: 30/11/2006
DOI: 10.1088/0957-4484/18/17/175302
URL: