Dr Nicholas Harmer

Associate Professor of Biochemistry

N.J.Harmer@exeter.ac.uk

CLES

Groups: Harmer Group

University profile

0000-0002-4073-0505

Publications

2023

The ROK kinase N-acetylglucosamine kinase uses a sequential random enzyme mechanism with successive conformational changes upon each substrate binding

Journal: Journal of Biological Chemistry

Published: 31/03/2023
DOI: 10.1016/j.jbc.2023.103033
URL: https://doi.org/10.1016/j.jbc.2023.103033

Fluorescent probe for the identification of potent inhibitors of the macrophage infectivity potentiator (Mip) protein of Burkholderia pseudomallei

Journal: SLAS Discovery

Published: 28/02/2023
DOI: 10.1016/j.slasd.2023.03.004
URL: https://doi.org/10.1016/j.slasd.2023.03.004

2022

Survivor bias drives overestimation of stability in reconstructed ancestral proteins

Published: 25/11/2022
DOI: 10.1101/2022.11.23.517659
URL: https://doi.org/10.1101/2022.11.23.517659

Towards Elucidating the Rotary Mechanism of the Archaellum Machinery

Journal: Frontiers in Microbiology

Published: 21/03/2022
DOI: 10.3389/fmicb.2022.848597
ISSN: 1664-302X
URL: http://dx.doi.org/10.3389/fmicb.2022.848597

Pseudomonas aeruginosa Biofilm Dispersion by the Human Atrial Natriuretic Peptide

Journal: Advanced Science

Published: 28/02/2022
DOI: 10.1002/advs.202103262
URL: https://doi.org/10.1002/advs.202103262

2021

Unique Data Sets and Bespoke Laboratory Videos: Teaching and Assessing of Experimental Methods and Data Analysis in a Pandemic

Journal: Journal of Chemical Education

Published: 14/12/2021
DOI: 10.1021/acs.jchemed.1c00853
URL: https://doi.org/10.1021/acs.jchemed.1c00853

Structure and function of N-acetylglucosamine kinase illuminates the catalytic mechanism of ROK kinases

Published: 30/09/2021
DOI: 10.1101/2021.09.30.462564
URL: https://doi.org/10.1101/2021.09.30.462564

Heterologous Protein Expression Favors the Formation of Protein Aggregates in Persister and Viable but Nonculturable Bacteria.

Journal: ACS Infectious Diseases

Published: 11/06/2021
DOI: 10.1021/acsinfecdis.1c00154
URL: https://doi.org/10.1021/acsinfecdis.1c00154

Drug screening to identify compounds to act as co-therapies for the treatment of Burkholderia species

Journal: PLOS ONE

Published: 25/03/2021
DOI: 10.1371/journal.pone.0248119
URL: https://doi.org/10.1371/journal.pone.0248119

2020

Special Issue on the 60th anniversary of the first laser—Series I: Microcavity Photonics—from fundamentals to applications

Journal: Light: Science and Applications

Published: 30/11/2020
DOI: 10.1038/s41377-021-00583-w
EID: 2-s2.0-85109707384
ISSN: 20477538 20955545
URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-85109707384&partnerID=MN8TOARS

Using enzyme cascades in biocatalysis: Highlight on transaminases and carboxylic acid reductases

Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Published: 31/01/2020
DOI: 10.1016/j.bbapap.2019.140322
URL: https://doi.org/10.1016/j.bbapap.2019.140322

2019

Highly thermostable carboxylic acid reductases generated by ancestral sequence reconstruction

Journal: Communications Biology

Published: 22/11/2019
DOI: 10.1038/s42003-019-0677-y
URL: https://doi.org/10.1038/s42003-019-0677-y

Molecular features of lipoprotein CD0873: A potential vaccine against the human pathogen Clostridioides difficile

Journal: Journal of Biological Chemistry

Published: 30/09/2019
DOI: 10.1074/jbc.RA119.010120
URL: https://doi.org/10.1074/jbc.RA119.010120

Engineering a Seven Enzyme Biotransformation using Mathematical Modelling and Characterized Enzyme Parts

Journal: ChemCatChem

Published: 07/08/2019
DOI: 10.1002/cctc.201900646
URL: https://doi.org/10.1002/cctc.201900646

Drug screening to identify compounds to act as co-therapies for the treatment of pathogenic Burkholderia

Published: 08/07/2019
DOI: 10.1101/692129
URL: https://doi.org/10.1101/692129

A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity

Published: 13/02/2019
DOI: 10.1101/548628
URL: https://doi.org/10.1101/548628

2018

Carbohydrate Kinases: A Conserved Mechanism Across Differing Folds

Journal: Catalysts

Published: 31/12/2018
DOI: 10.3390/catal9010029
URL: http://www.mdpi.com/2073-4344/9/1/29

Pair creation, motion, and annihilation of topological defects in two-dimensional nematic liquid crystals

Journal: Physical Review E

Published: 31/01/2018
DOI: 10.1103/physreve.97.022704
ISSN: 2470-0045
URL:

2017

A half-site multimeric enzyme achieves its cooperativity without conformational changes

Journal: Scientific Reports

Published: 28/11/2017
DOI: 10.1038/s41598-017-16421-2
URL: https://doi.org/10.1038/s41598-017-16421-2

Characterization of Carboxylic Acid Reductases as Enzymes in the Toolbox for Synthetic Chemistry

Journal: ChemCatChem

Published: 20/03/2017
DOI: 10.1002/cctc.201601249
URL:

2014

Achieving high signal-to-noise in cell regulatory systems: Spatial organization of multiprotein transmembrane assemblies of FGFR and MET receptors

Published: 30/11/2014
DOI: 10.1016/j.pbiomolbio.2015.04.007
URL:

Pseudomonas aeruginosa Expresses a Functional Human Natriuretic Peptide Receptor Ortholog: Involvement in Biofilm Formation

Published: 30/11/2014
DOI: 10.1128/mBio.01033-15
URL:

Unravelling the B. pseudomallei heptokinase WcbL: from Structure to Drug Discovery

Journal: Chemistry & Biology

Published: 30/11/2014
DOI: 10.1016/j.chembiol.2015.10.015
URL:

2013

A structural biology approach enables to development of antimicrobials targeting bacterial immunophilins

Journal: Antimicrobial Agents and Chemotherapy

Published: 30/11/2013
DOI: 10.1128/AAC.01875-13
URL:

Determination of protein-ligand interactions using differential scanning fluorimetry

Published: 30/11/2013
DOI: 10.3791/51809
URL:

Structural insights into WcbI, a novel polysaccharide-biosynthesis enzyme

Journal: IUCrJ

Published: 30/11/2013
DOI: 10.1107/S205225251302695X
URL:

The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation

Journal: Biochemical Journal

Published: 30/11/2013
DOI: 10.1042/BJ20140073
URL:

2012

Identification of type II toxin-antitoxin modules inBurkholderia pseudomallei

Published: 30/11/2012
ISSN: 0378-1097
DOI: 10.1111/1574-6968.12032
URL:

Photoinduced modification of component composition of molecular layers with silver nanoparticles

Journal: Fundamentals of Laser-Assisted Micro- and Nanotechnologies 2013

Published: 30/11/2012
DOI: 10.1117/12.2052535
WOSUID: WOS:000333048100032
URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000333048100032&KeyUID=WOS:000333048100032

2011

Phosphate transporters in marine phytoplankton and their viruses: cross-domain commonalities in viral-host gene exchanges

Published: 30/11/2011
DOI: 10.1111/j.1462-2920.2011.02576.x
ISSN: 1462-2912
URL:

2010

Myosin-5, kinesin-1 and myosin-17 cooperate in secretion of fungal chitin synthase

Published: 30/11/2010
ISSN: 0261-4189
DOI: 10.1038/emboj.2011.361
URL:

The structure of aBurkholderia pseudomalleiimmunophilin–inhibitor complex reveals new approaches to antimicrobial development

Published: 30/11/2010
ISSN: 0264-6021
DOI: 10.1042/bj20110345
URL:

2009

The Structure of Sedoheptulose-7-Phosphate Isomerase from Burkholderia pseudomallei Reveals a Zinc Binding Site at the Heart of the Active Site

Published: 30/11/2009
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2010.04.058
URL:

Backtracking and proofreading in DNA transcription.

Published: 31/05/2009
PMID: 19659121
DOI: 10.1103/physrevlett.102.258101
URL: http://europepmc.org/abstract/med/19659121

2006

Cloning, expression, purification and preliminary crystallographic analysis of the short-chain dehydrogenase enzymes WbmF, WbmG and WbmH fromBordetella bronchiseptica

Published: 30/11/2006
DOI: 10.1107/s174430910703477x
ISSN: 1744-3091
URL:

Predicting Protein Function from Structure—The Roles of Short-chain Dehydrogenase/Reductase Enzymes in Bordetella O-antigen Biosynthesis

Published: 30/11/2006
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2007.09.055
URL:

2005

Expression and purification of recombinant human fibroblast growth factor receptor in Escherichia coli

Published: 30/11/2005
DOI: 10.1016/j.pep.2006.04.008
ISSN: 1046-5928
URL:

Multimers of the fibroblast growth factor (FGF)–FGF receptor–saccharide complex are formed on long oligomers of heparin

Published: 30/11/2005
ISSN: 0264-6021
DOI: 10.1042/bj20050985
URL:

2004

1.15Å Crystal structure of the X. tropicalis Spred1 EVH1 domain suggests a fourth distinct peptide-binding mechanism within the EVH1 family

Published: 30/11/2004
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2004.11.114
URL:

Role of Heparan Sulfate in Fibroblast Growth Factor Signaling

Published: 30/11/2004
DOI: 10.1016/b978-008044859-6/50015-0
ISBN: 9780080448596
URL:

2003

British Society for Matrix Biology Meeting

Published: 30/11/2003
ISSN: 0959-9673
DOI: 10.1111/j.0959-9673.2004.390ak.x
URL:

The Crystal Structure of Fibroblast Growth Factor (FGF) 19 Reveals Novel Features of the FGF Family and Offers a Structural Basis for Its Unusual Receptor Affinity†,‡

Published: 30/11/2003
ISSN: 0006-2960
DOI: 10.1021/bi035320k
URL:

Towards a Resolution of the Stoichiometry of the Fibroblast Growth Factor (FGF)–FGF Receptor–Heparin Complex

Published: 30/11/2003
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2004.04.031
URL:

2002

The structural biology of growth factor receptor activation

Published: 30/11/2002
ISSN: 0301-4622
DOI: 10.1016/s0301-4622(02)00305-8
URL:

2000

Sequence analyses and comparative modeling of fly and worm fibroblast growth factor receptors indicate that the determinants for FGF and heparin binding are retained in evolution

Published: 30/11/2000
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(01)02603-5
URL: