Dr Nicholas Harmer Associate Professor of Biochemistry N.J.Harmer@exeter.ac.uk CLES Groups: Harmer Group University profile 0000-0002-4073-0505 Publications Publications 2023 A Murder Mystery Gamification Session to Consolidate Analytical Biochemical Techniques Learning Journal: Journal of Chemical Education Published: 14/11/2023 DOI: 10.1021/acs.jchemed.3c00279 URL: https://doi.org/10.1021/acs.jchemed.3c00279 Structural insights into the DNA recognition mechanism by the bacterial transcription factor PdxR Journal: Nucleic Acids Research Published: 25/08/2023 DOI: 10.1093/nar/gkad552 URL: https://doi.org/10.1093/nar/gkad552 Fluorescent probe for the identification of potent inhibitors of the macrophage infectivity potentiator (Mip) protein of Burkholderia pseudomallei Journal: SLAS Discovery Published: 30/06/2023 DOI: 10.1016/j.slasd.2023.03.004 URL: https://doi.org/10.1016/j.slasd.2023.03.004 The ROK kinase N-acetylglucosamine kinase uses a sequential random enzyme mechanism with successive conformational changes upon each substrate binding Journal: Journal of Biological Chemistry Published: 31/03/2023 DOI: 10.1016/j.jbc.2023.103033 URL: https://doi.org/10.1016/j.jbc.2023.103033 2022 Survivor bias drives overestimation of stability in reconstructed ancestral proteins Published: 25/11/2022 DOI: 10.1101/2022.11.23.517659 URL: https://doi.org/10.1101/2022.11.23.517659 Towards Elucidating the Rotary Mechanism of the Archaellum Machinery Journal: Frontiers in Microbiology Published: 21/03/2022 DOI: 10.3389/fmicb.2022.848597 ISSN: 1664-302X URL: http://dx.doi.org/10.3389/fmicb.2022.848597 Pseudomonas aeruginosa Biofilm Dispersion by the Human Atrial Natriuretic Peptide Journal: Advanced Science Published: 28/02/2022 DOI: 10.1002/advs.202103262 URL: https://doi.org/10.1002/advs.202103262 2021 Unique Data Sets and Bespoke Laboratory Videos: Teaching and Assessing of Experimental Methods and Data Analysis in a Pandemic Journal: Journal of Chemical Education Published: 14/12/2021 DOI: 10.1021/acs.jchemed.1c00853 URL: https://doi.org/10.1021/acs.jchemed.1c00853 Structure and function of N-acetylglucosamine kinase illuminates the catalytic mechanism of ROK kinases Published: 30/09/2021 DOI: 10.1101/2021.09.30.462564 URL: https://doi.org/10.1101/2021.09.30.462564 Heterologous Protein Expression Favors the Formation of Protein Aggregates in Persister and Viable but Nonculturable Bacteria. Journal: ACS Infectious Diseases Published: 11/06/2021 DOI: 10.1021/acsinfecdis.1c00154 URL: https://doi.org/10.1021/acsinfecdis.1c00154 Drug screening to identify compounds to act as co-therapies for the treatment of Burkholderia species Journal: PLOS ONE Published: 25/03/2021 DOI: 10.1371/journal.pone.0248119 URL: https://doi.org/10.1371/journal.pone.0248119 2020 Special Issue on the 60th anniversary of the first laser—Series I: Microcavity Photonics—from fundamentals to applications Journal: Light: Science and Applications Published: 30/11/2020 DOI: 10.1038/s41377-021-00583-w EID: 2-s2.0-85109707384 ISSN: 20477538 20955545 URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-85109707384&partnerID=MN8TOARS Using enzyme cascades in biocatalysis: Highlight on transaminases and carboxylic acid reductases Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics Published: 31/01/2020 DOI: 10.1016/j.bbapap.2019.140322 URL: https://doi.org/10.1016/j.bbapap.2019.140322 2019 Highly thermostable carboxylic acid reductases generated by ancestral sequence reconstruction Journal: Communications Biology Published: 22/11/2019 DOI: 10.1038/s42003-019-0677-y URL: https://doi.org/10.1038/s42003-019-0677-y Molecular features of lipoprotein CD0873: A potential vaccine against the human pathogen Clostridioides difficile Journal: Journal of Biological Chemistry Published: 30/09/2019 DOI: 10.1074/jbc.RA119.010120 URL: https://doi.org/10.1074/jbc.RA119.010120 Engineering a Seven Enzyme Biotransformation using Mathematical Modelling and Characterized Enzyme Parts Journal: ChemCatChem Published: 07/08/2019 DOI: 10.1002/cctc.201900646 URL: https://doi.org/10.1002/cctc.201900646 Drug screening to identify compounds to act as co-therapies for the treatment of pathogenic Burkholderia Published: 08/07/2019 DOI: 10.1101/692129 URL: https://doi.org/10.1101/692129 A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity Published: 13/02/2019 DOI: 10.1101/548628 URL: https://doi.org/10.1101/548628 2018 Carbohydrate Kinases: A Conserved Mechanism Across Differing Folds Journal: Catalysts Published: 31/12/2018 DOI: 10.3390/catal9010029 URL: http://www.mdpi.com/2073-4344/9/1/29 Pair creation, motion, and annihilation of topological defects in two-dimensional nematic liquid crystals Journal: Physical Review E Published: 31/01/2018 DOI: 10.1103/physreve.97.022704 ISSN: 2470-0045 URL: 2017 A half-site multimeric enzyme achieves its cooperativity without conformational changes Journal: Scientific Reports Published: 28/11/2017 DOI: 10.1038/s41598-017-16421-2 URL: https://doi.org/10.1038/s41598-017-16421-2 Characterization of Carboxylic Acid Reductases as Enzymes in the Toolbox for Synthetic Chemistry Journal: ChemCatChem Published: 20/03/2017 DOI: 10.1002/cctc.201601249 URL: https://doi.org/10.1002/cctc.201601249 2014 Achieving high signal-to-noise in cell regulatory systems: Spatial organization of multiprotein transmembrane assemblies of FGFR and MET receptors Published: 30/11/2014 DOI: 10.1016/j.pbiomolbio.2015.04.007 URL: Pseudomonas aeruginosa Expresses a Functional Human Natriuretic Peptide Receptor Ortholog: Involvement in Biofilm Formation Published: 30/11/2014 DOI: 10.1128/mBio.01033-15 URL: Unravelling the B. pseudomallei heptokinase WcbL: from Structure to Drug Discovery Journal: Chemistry & Biology Published: 30/11/2014 DOI: 10.1016/j.chembiol.2015.10.015 URL: 2013 A structural biology approach enables to development of antimicrobials targeting bacterial immunophilins Journal: Antimicrobial Agents and Chemotherapy Published: 30/11/2013 DOI: 10.1128/AAC.01875-13 URL: Determination of protein-ligand interactions using differential scanning fluorimetry Published: 30/11/2013 DOI: 10.3791/51809 URL: Structural insights into WcbI, a novel polysaccharide-biosynthesis enzyme Journal: IUCrJ Published: 30/11/2013 DOI: 10.1107/S205225251302695X URL: The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation Journal: Biochemical Journal Published: 30/11/2013 DOI: 10.1042/BJ20140073 URL: 2012 Identification of type II toxin-antitoxin modules inBurkholderia pseudomallei Published: 30/11/2012 ISSN: 0378-1097 DOI: 10.1111/1574-6968.12032 URL: Photoinduced modification of component composition of molecular layers with silver nanoparticles Journal: Fundamentals of Laser-Assisted Micro- and Nanotechnologies 2013 Published: 30/11/2012 DOI: 10.1117/12.2052535 WOSUID: WOS:000333048100032 URL: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000333048100032&KeyUID=WOS:000333048100032 2011 Phosphate transporters in marine phytoplankton and their viruses: cross-domain commonalities in viral-host gene exchanges Published: 30/11/2011 DOI: 10.1111/j.1462-2920.2011.02576.x ISSN: 1462-2912 URL: 2010 Myosin-5, kinesin-1 and myosin-17 cooperate in secretion of fungal chitin synthase Published: 30/11/2010 ISSN: 0261-4189 DOI: 10.1038/emboj.2011.361 URL: The structure of aBurkholderia pseudomalleiimmunophilin–inhibitor complex reveals new approaches to antimicrobial development Published: 30/11/2010 ISSN: 0264-6021 DOI: 10.1042/bj20110345 URL: 2009 The Structure of Sedoheptulose-7-Phosphate Isomerase from Burkholderia pseudomallei Reveals a Zinc Binding Site at the Heart of the Active Site Published: 30/11/2009 ISSN: 0022-2836 DOI: 10.1016/j.jmb.2010.04.058 URL: Backtracking and proofreading in DNA transcription. Published: 31/05/2009 PMID: 19659121 DOI: 10.1103/physrevlett.102.258101 URL: http://europepmc.org/abstract/med/19659121 2006 Cloning, expression, purification and preliminary crystallographic analysis of the short-chain dehydrogenase enzymes WbmF, WbmG and WbmH fromBordetella bronchiseptica Published: 30/11/2006 DOI: 10.1107/s174430910703477x ISSN: 1744-3091 URL: Predicting Protein Function from Structure—The Roles of Short-chain Dehydrogenase/Reductase Enzymes in Bordetella O-antigen Biosynthesis Published: 30/11/2006 ISSN: 0022-2836 DOI: 10.1016/j.jmb.2007.09.055 URL: 2005 Expression and purification of recombinant human fibroblast growth factor receptor in Escherichia coli Published: 30/11/2005 DOI: 10.1016/j.pep.2006.04.008 ISSN: 1046-5928 URL: Multimers of the fibroblast growth factor (FGF)–FGF receptor–saccharide complex are formed on long oligomers of heparin Published: 30/11/2005 ISSN: 0264-6021 DOI: 10.1042/bj20050985 URL: 2004 1.15Å Crystal structure of the X. tropicalis Spred1 EVH1 domain suggests a fourth distinct peptide-binding mechanism within the EVH1 family Published: 30/11/2004 ISSN: 0014-5793 DOI: 10.1016/j.febslet.2004.11.114 URL: Role of Heparan Sulfate in Fibroblast Growth Factor Signaling Published: 30/11/2004 DOI: 10.1016/b978-008044859-6/50015-0 ISBN: 9780080448596 URL: 2003 British Society for Matrix Biology Meeting Published: 30/11/2003 ISSN: 0959-9673 DOI: 10.1111/j.0959-9673.2004.390ak.x URL: The Crystal Structure of Fibroblast Growth Factor (FGF) 19 Reveals Novel Features of the FGF Family and Offers a Structural Basis for Its Unusual Receptor Affinity†,‡ Published: 30/11/2003 ISSN: 0006-2960 DOI: 10.1021/bi035320k URL: Towards a Resolution of the Stoichiometry of the Fibroblast Growth Factor (FGF)–FGF Receptor–Heparin Complex Published: 30/11/2003 ISSN: 0022-2836 DOI: 10.1016/j.jmb.2004.04.031 URL: 2002 The structural biology of growth factor receptor activation Published: 30/11/2002 ISSN: 0301-4622 DOI: 10.1016/s0301-4622(02)00305-8 URL: 2000 Sequence analyses and comparative modeling of fly and worm fibroblast growth factor receptors indicate that the determinants for FGF and heparin binding are retained in evolution Published: 30/11/2000 ISSN: 0014-5793 DOI: 10.1016/s0014-5793(01)02603-5 URL: